|
KMID : 1134820200490030289
|
|
Journal of the Korean Society of Food Science and Nutrition
2020 Volume.49 No. 3 p.289 ~ p.294
|
|
|
Anti-Inflammatory Activities of Hot Water Extracts of Petasites japonicus Leaves in LPS-Induced RAW264.7 Cells
|
|
Kim Eun-Jin
Kim Han-Na
Kim Hae-Mi
Park Sang-Su
|
|
|
Abstract
|
|
|
|
Petasites japonicus has been widely used as a medicinal herb in Korea, Japan, and China. This study investigated the anti-inflammatory activity of hot water extracts of P. japonicus leaves (KP-1) on lipopolysaccharide (LPS)-induced RAW264.7 cells. LPS stimulates the differentiation of monocytes into macrophages and it induces the pro-inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), reactive oxygen species (ROS), and cytokines. The results determined that KP-1 did not influence the cytotoxicity of macrophages at 25, 50, and 100 μg/mL. Also, pre-treatment with KP-1 significantly reduced the production of NO and PGE2, and dose-dependently attenuated the ROS levels in the LPS-stimulated cells. In addition, the study evaluated whether fukinolic acid (FA) isolated from KP-1 contributes to the anti-inflammatory properties of KP-1. As a result, FA treatment showed a tendency toward decreased NO production, and inhibited the generation of ROS in activated macrophages. The results suggest that KP-1 has anti-inflammatory activity on the LPS-mediated inflammatory response and may be suitable for the development of potential functional foods.
|
|
|
KEYWORD
|
|
|
Petasites japonicus, anti-inflammatory activity, macrophages, fukinolic acid
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|